tph2 gfp Search Results


rabbit anti-tph2  (Novus Biologicals)
90
Novus Biologicals rabbit anti-tph2
Rabbit Anti Tph2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-tph2/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
rabbit anti-tph2 - by Bioz Stars, 2026-02
90/100 stars
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af488 donkey α rabbit  (Thermo Fisher)
86
Thermo Fisher af488 donkey α rabbit
(A) Mouse <t>TPH2-CreERT2</t> expression cassette for DNA microinjection. (B–I) DAB-immunohistochemistry with a Cre antibody shows weak Cre expression in the brain stem and mid-brain of TPH2-CreERT2 rat founder lines #7 ( B,C), #8 ( D,E), and #14 ( F,G). Founder line #15 shows extensive Cre staining in areas where serotonergic raphe nuclei are located ( H,I). Intensity of Cre expression correlates with the transgene copy number of TPH2-CreERT2 rat founders ( J).
Af488 Donkey α Rabbit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af488 donkey α rabbit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
af488 donkey α rabbit - by Bioz Stars, 2026-02
86/100 stars
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tph2 gfp  (Addgene inc)
93
Addgene inc tph2 gfp
(A) HTR6 (labeled by CF633, green in merged panel) is highly enriched in CA1 neuronal primary cilia (ADCY3, magenta in the merged panel: 20 μm MIP). (B) Magnified images from (A). 5-HTR6s are not evenly distributed along the length of cilia and can be enriched at areas with low ADCY3 labeling (arrow). (C) Left panel: cilia (magenta) co-labeled with serotonergic axons (green). 20-μm maximum intensity projection (MIP). Middle panel: cilia in the left panel color coded by the shortest distance to a serotonergic axon. Right panel: cilia from left panel color coded with the shortest distance to a serotonergic axon-associated synaptophysin punctum. (D) Floxed synaptophysin-EGFP driven by <t>Tph2</t> -Cre showed ADCY3-labeled cilia (magenta in the merged panel) in contact with serotonergic presynaptic terminals (amplified by <t>anti-GFP</t> and Alexa 488, green in the merged panel). (E) 5-HTR6 (green in the merged panel) are enriched on the cilia at the axonal contact sites (SERT, cyan in the merged panel). Two cilia are in contact with a single serotonergic axonal varicosity. ADCY3 (magenta in the merged panel) can extend beyond the contact site that has few 5-HTR6 (arrow). (A), (B), and (E) are deconvolved confocal images with photon counting detection (Leica). (C) and (D) are Airyscan images (Zeiss). Data from 3- to 4-month-old male C57BL/6J mice.
Tph2 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tph2 gfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
tph2 gfp - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


(A) Mouse TPH2-CreERT2 expression cassette for DNA microinjection. (B–I) DAB-immunohistochemistry with a Cre antibody shows weak Cre expression in the brain stem and mid-brain of TPH2-CreERT2 rat founder lines #7 ( B,C), #8 ( D,E), and #14 ( F,G). Founder line #15 shows extensive Cre staining in areas where serotonergic raphe nuclei are located ( H,I). Intensity of Cre expression correlates with the transgene copy number of TPH2-CreERT2 rat founders ( J).

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A) Mouse TPH2-CreERT2 expression cassette for DNA microinjection. (B–I) DAB-immunohistochemistry with a Cre antibody shows weak Cre expression in the brain stem and mid-brain of TPH2-CreERT2 rat founder lines #7 ( B,C), #8 ( D,E), and #14 ( F,G). Founder line #15 shows extensive Cre staining in areas where serotonergic raphe nuclei are located ( H,I). Intensity of Cre expression correlates with the transgene copy number of TPH2-CreERT2 rat founders ( J).

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Expressing, Immunohistochemistry, Staining

(A,C,E,G) DAB-immunohistochemistry with a Cre antibody of line #15 shows Cre staining in the brain stem and mid-brain, regions which contain serotonergic somata while extraserotonergic brain regions show no staining. (B,D,F,H) Coronal sections of dual-label fluorescence immunohistochemistry with Cre and TPH1 antibodies. The TPH1 antibody crossreacts with TPH2 and detects both isoenzymes. Colocalization of TPH1 and Cre confirms exclusive Cre expression in 5-HT neurons of the raphe nuclei. Caudal raphe nuclei (CR); dorsal raphe nuclei (DR); median raphe nuclei (MR). Scale bars: 100 µm.

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A,C,E,G) DAB-immunohistochemistry with a Cre antibody of line #15 shows Cre staining in the brain stem and mid-brain, regions which contain serotonergic somata while extraserotonergic brain regions show no staining. (B,D,F,H) Coronal sections of dual-label fluorescence immunohistochemistry with Cre and TPH1 antibodies. The TPH1 antibody crossreacts with TPH2 and detects both isoenzymes. Colocalization of TPH1 and Cre confirms exclusive Cre expression in 5-HT neurons of the raphe nuclei. Caudal raphe nuclei (CR); dorsal raphe nuclei (DR); median raphe nuclei (MR). Scale bars: 100 µm.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Immunohistochemistry, Staining, Fluorescence, Expressing

(A,B) X-Gal staining of sagittal sections shows ubiquitous βgal activity throughout the brain of adult CAG-loxP.EGFP rats (P90). (C–K) Dual-label fluorescence immunohistochemistry (IHC). (C–E) βgal/NeuN IHC of the cerebellum (C), cortex (D) and OB (E) shows strong colocalization of βgal with the neuronal marker NeuN. (F–H) βgal IHC with the serotonergic marker TPH2 (F), and the dopaminergic and noradrenergic marker tyrosine hydroxylase (TH) (G,H) shows abundant colocalization of βgal with 5-HT neurons in the dorsal raphe (F), with dopaminergic neurons in the ventral tegmental area and substantia nigra (G) and noradrenergic neurons in the locus coeruleus (H) confirming strong βgal expression in all monoaminergic neurons. (I,J) βgal/GAD67 IHC shows βgal expression in GABAergic neurons of the granular layer of the OB (I) and in the hippocampus (J). (K) βgal/GFAP IHC in the hippocampus shows infrequent βgal expression in glia. OB, olfactory bulb; DR, dorsal raphe nuclei; VTA, ventral tegmental area; SN, substantia nigra; LC, locus coeruleus; HC, hippocampus. Scale bars: 100 µm.

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A,B) X-Gal staining of sagittal sections shows ubiquitous βgal activity throughout the brain of adult CAG-loxP.EGFP rats (P90). (C–K) Dual-label fluorescence immunohistochemistry (IHC). (C–E) βgal/NeuN IHC of the cerebellum (C), cortex (D) and OB (E) shows strong colocalization of βgal with the neuronal marker NeuN. (F–H) βgal IHC with the serotonergic marker TPH2 (F), and the dopaminergic and noradrenergic marker tyrosine hydroxylase (TH) (G,H) shows abundant colocalization of βgal with 5-HT neurons in the dorsal raphe (F), with dopaminergic neurons in the ventral tegmental area and substantia nigra (G) and noradrenergic neurons in the locus coeruleus (H) confirming strong βgal expression in all monoaminergic neurons. (I,J) βgal/GAD67 IHC shows βgal expression in GABAergic neurons of the granular layer of the OB (I) and in the hippocampus (J). (K) βgal/GFAP IHC in the hippocampus shows infrequent βgal expression in glia. OB, olfactory bulb; DR, dorsal raphe nuclei; VTA, ventral tegmental area; SN, substantia nigra; LC, locus coeruleus; HC, hippocampus. Scale bars: 100 µm.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Staining, Activity Assay, Fluorescence, Immunohistochemistry, Marker, Expressing

(A) TPH2-CreERT2 rats were bred to CAG-loxP.EGFP rats to generate double-transgenic TPH2-CreERT2/CAG-loxP.EGFP rats. Under uninduced baseline conditions, the loxP-flanked lacZ minigene is expressed reflecting cell-type specific CAG-promoter activity. Upon Cre-mediated recombination (+ Tamoxifen), lacZ is replaced with the second reporter gene enhanced green fluorescent protein (EGFP). The appearance of EGFP serves as an indicator of Cre mediated recombination in double transgenic rats. TPH2-CreERT2/CAG-loxP.EGFP rats were daily injected with tamoxifen (40 mg/kg) or vehicle for five consecutive days starting between P60–90. Coronal sections show dual-label fluorescence immunohistochemistry for TPH/βgal (B,E,H,K) and TPH/GFP (C,F,I,L) in vehicle-treated rats (-Tx) and TPH/GFP in tamoxifen-treated (+Tx) rats (D,G,J,M). Colocalization is visualized at the level of caudal raphe nuclei (CR) (B–D), dorsal raphe nuclei (DR) (E–J) and median raphe nuclei (MR) (K–M) using confocal images. In vehicle-treated rats, TPH2-CreERT2/CAG-loxP.EGFP rats display strong basal, non-recombined βgal expression in TPH2+ 5-HT neurons (B,E,H,K) making these rats ideally suited to monitor tamoxifen-induced Cre-mediated recombination in 5-HT neurons. (C,F,I,L) Without tamoxifen treatment, background recombination, i.e. EGFP expression (arrows) hardly occurs. (D,G,J,M) After tamoxifen treatment, the majority of TPH+ 5-HT neurons in all raphe nuclei now show EGFP expression indicating Cre-mediated recombination in these neurons (GFP+/TPH+). Scale bars: 100 µm.

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A) TPH2-CreERT2 rats were bred to CAG-loxP.EGFP rats to generate double-transgenic TPH2-CreERT2/CAG-loxP.EGFP rats. Under uninduced baseline conditions, the loxP-flanked lacZ minigene is expressed reflecting cell-type specific CAG-promoter activity. Upon Cre-mediated recombination (+ Tamoxifen), lacZ is replaced with the second reporter gene enhanced green fluorescent protein (EGFP). The appearance of EGFP serves as an indicator of Cre mediated recombination in double transgenic rats. TPH2-CreERT2/CAG-loxP.EGFP rats were daily injected with tamoxifen (40 mg/kg) or vehicle for five consecutive days starting between P60–90. Coronal sections show dual-label fluorescence immunohistochemistry for TPH/βgal (B,E,H,K) and TPH/GFP (C,F,I,L) in vehicle-treated rats (-Tx) and TPH/GFP in tamoxifen-treated (+Tx) rats (D,G,J,M). Colocalization is visualized at the level of caudal raphe nuclei (CR) (B–D), dorsal raphe nuclei (DR) (E–J) and median raphe nuclei (MR) (K–M) using confocal images. In vehicle-treated rats, TPH2-CreERT2/CAG-loxP.EGFP rats display strong basal, non-recombined βgal expression in TPH2+ 5-HT neurons (B,E,H,K) making these rats ideally suited to monitor tamoxifen-induced Cre-mediated recombination in 5-HT neurons. (C,F,I,L) Without tamoxifen treatment, background recombination, i.e. EGFP expression (arrows) hardly occurs. (D,G,J,M) After tamoxifen treatment, the majority of TPH+ 5-HT neurons in all raphe nuclei now show EGFP expression indicating Cre-mediated recombination in these neurons (GFP+/TPH+). Scale bars: 100 µm.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Transgenic Assay, Activity Assay, Injection, Fluorescence, Immunohistochemistry, Expressing

Recombination efficacy and background recombination for rat TPH2-CreERT2 line #15.

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: Recombination efficacy and background recombination for rat TPH2-CreERT2 line #15.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques:

(A) HTR6 (labeled by CF633, green in merged panel) is highly enriched in CA1 neuronal primary cilia (ADCY3, magenta in the merged panel: 20 μm MIP). (B) Magnified images from (A). 5-HTR6s are not evenly distributed along the length of cilia and can be enriched at areas with low ADCY3 labeling (arrow). (C) Left panel: cilia (magenta) co-labeled with serotonergic axons (green). 20-μm maximum intensity projection (MIP). Middle panel: cilia in the left panel color coded by the shortest distance to a serotonergic axon. Right panel: cilia from left panel color coded with the shortest distance to a serotonergic axon-associated synaptophysin punctum. (D) Floxed synaptophysin-EGFP driven by Tph2 -Cre showed ADCY3-labeled cilia (magenta in the merged panel) in contact with serotonergic presynaptic terminals (amplified by anti-GFP and Alexa 488, green in the merged panel). (E) 5-HTR6 (green in the merged panel) are enriched on the cilia at the axonal contact sites (SERT, cyan in the merged panel). Two cilia are in contact with a single serotonergic axonal varicosity. ADCY3 (magenta in the merged panel) can extend beyond the contact site that has few 5-HTR6 (arrow). (A), (B), and (E) are deconvolved confocal images with photon counting detection (Leica). (C) and (D) are Airyscan images (Zeiss). Data from 3- to 4-month-old male C57BL/6J mice.

Journal: Cell

Article Title: A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility

doi: 10.1016/j.cell.2022.07.026

Figure Lengend Snippet: (A) HTR6 (labeled by CF633, green in merged panel) is highly enriched in CA1 neuronal primary cilia (ADCY3, magenta in the merged panel: 20 μm MIP). (B) Magnified images from (A). 5-HTR6s are not evenly distributed along the length of cilia and can be enriched at areas with low ADCY3 labeling (arrow). (C) Left panel: cilia (magenta) co-labeled with serotonergic axons (green). 20-μm maximum intensity projection (MIP). Middle panel: cilia in the left panel color coded by the shortest distance to a serotonergic axon. Right panel: cilia from left panel color coded with the shortest distance to a serotonergic axon-associated synaptophysin punctum. (D) Floxed synaptophysin-EGFP driven by Tph2 -Cre showed ADCY3-labeled cilia (magenta in the merged panel) in contact with serotonergic presynaptic terminals (amplified by anti-GFP and Alexa 488, green in the merged panel). (E) 5-HTR6 (green in the merged panel) are enriched on the cilia at the axonal contact sites (SERT, cyan in the merged panel). Two cilia are in contact with a single serotonergic axonal varicosity. ADCY3 (magenta in the merged panel) can extend beyond the contact site that has few 5-HTR6 (arrow). (A), (B), and (E) are deconvolved confocal images with photon counting detection (Leica). (C) and (D) are Airyscan images (Zeiss). Data from 3- to 4-month-old male C57BL/6J mice.

Article Snippet: Tph2 -Cre vector was generated by replacing GFP in Tph2 -GFP( ; gift from Dr. Björklund) with the Cre-recombinase in Syn1-EBFP-Cre AAV (gift from Hongkui Zeng, Addgene plasmid # 51507; RRID:Addgene_51507). pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] was a gift from Edward Boyden (Addgene plasmid # 62723; RRID:Addgene_62723). pAAV[FLEX-ON]-CAG-Farnesylated-SNAPtag AAV plasmid was made by VectorBuilder, Inc. pAAV-FLEX-ON-tdTomato was a gift from Edward Boyden (Addgene plasmid # 28306; RRID:Addgene_28306). pAAV-EF1a-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (Addgene plasmid # 50460; RRID:Addgene_50460). pAAV-TRE-HTR6-SNAPf-TRIP and pAAV-Syn-Tet3G were made by VectorBuilder.

Techniques: Labeling, Amplification

KEY RESOURCES TABLE

Journal: Cell

Article Title: A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility

doi: 10.1016/j.cell.2022.07.026

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Tph2 -Cre vector was generated by replacing GFP in Tph2 -GFP( ; gift from Dr. Björklund) with the Cre-recombinase in Syn1-EBFP-Cre AAV (gift from Hongkui Zeng, Addgene plasmid # 51507; RRID:Addgene_51507). pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] was a gift from Edward Boyden (Addgene plasmid # 62723; RRID:Addgene_62723). pAAV[FLEX-ON]-CAG-Farnesylated-SNAPtag AAV plasmid was made by VectorBuilder, Inc. pAAV-FLEX-ON-tdTomato was a gift from Edward Boyden (Addgene plasmid # 28306; RRID:Addgene_28306). pAAV-EF1a-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (Addgene plasmid # 50460; RRID:Addgene_50460). pAAV-TRE-HTR6-SNAPf-TRIP and pAAV-Syn-Tet3G were made by VectorBuilder.

Techniques: Virus, Recombinant, Knock-In, Software